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Preface<br> <br> PART I: Basic Methodology<br> <br> INFRARED AND RAMAN INSTRUMENTATION FOR MAPPING AND IMAGING<br> Introduction to Mapping and Imaging<br> Mid-Infrared Microspectroscopy and Mapping<br> Raman Microspectroscopy and Mapping<br> Near-Infrared Hyperspectral Imaging<br> Raman Hyperspectral Imaging<br> Mid-Infrared Hyperspectral Imaging<br> Mapping with Pulsed Terahertz Radiation<br> Summary<br> <br> CHEMOMETRIC TOOLS FOR IMAGE ANALYSIS<br> Introduction<br> Hyperspectral Images: The Measurement<br> Image Preprocessing<br> Exploratory Image Analysis<br> Quantitative Image Information: Multivariate Image Regression (MIR)<br> Image Segmentation<br> Image Resolution<br> Future Trends<br> <br> PART II: Biomedical Applications<br> <br> VIBRATIONAL SPECTROSCOPIC IMAGING OF SOFT TISSUE<br> Introduction<br> Preparation of Soft Tissue for Vibrational Spectroscopic Imaging<br> Applications to Soft Tissue<br> Conclusions<br> <br> VIBRATIONAL SPECTROSCOPIC ANALYSIS OF HARD TISSUES<br> Introduction<br> Importance of Tissue Age versus Specimen Age<br> FT-IR Spectroscopy<br> Raman Spectroscopy<br> Clinical Applications of Raman Spectroscopy<br> <br> MEDICAL APPLICATIONS OF INFRARED SPECTRAL IMAGING OF INDIVIDUAL CELLS<br> Introduction<br> Methods<br> Results and Discussion<br> Future Potential of SCP/Conclusions<br> <br> PART III: Agriculture, Plants, and Food<br> <br> INFRARED AND RAMAN SPECTROSCOPIC MAPPING AND IMAGING OF PLANT MATERIALS<br> Introduction, Background, and Perspective<br> Application of Mapping and Imaging to Horticultural Crops<br> Application of Mapping and Imaging to Agricultural Crops<br> Mapping and Imaging of Wild Plants and Trees<br> Application of Mapping and Imaging to Algae<br> Interaction Between Plant Tissue and Plant Pathogens<br> <br> NIR HYPERSPECTRAL IMAGING FOR FOOD AND AGRICULTURAL PRODUCTS<br> Introduction<br> HSI as a "Super" NIR Analyzer<br> NIR HS Imager as a "Super" Vision System<br> Conclusion<br> <br> PART IV: Polymers and Pharmaceuticals<br> <br> FT-IR AND NIR SPECTROSCOPIC IMAGING: PRINCIPLES, PRACTICAL ASPECTS, AND APPLICATIONS IN MATERIAL AND PHARMACEUTICAL SCIENCE<br> Introduction<br> Instrumentation for NIR and FT-IR Imaging<br> Applications of FT-IR and FT-NIR Imaging for Polymer Characterization<br> NIR Imaging Spectroscopy for Quality Control of Pharmaceutical Drug Formulations<br> FT-IR Spectroscopic Imaging of Inorganic Materials<br> <br> FT-IR IMAGING IN ATR AND TRANSMISSION MODES: PRACTCAL CONSIDERATIONS AND EMERGING APPLICATIONS<br> FT-IR Imaging: Introduction<br> FT-IR Imaging: Technical Considerations<br> Practical Applications<br> Conclusion and Outlook<br> <br> TERAHERTZ IMAGING OF DRUG PRODUCTS<br> Introduction<br> Low Wavenumber Region in the Infrared Spectrum<br> THz-TDS Technology and Applications<br> THz Imaging in the Pharmaceutical Industry<br> Going Forward<br> Competition versus Cost: A Challenge for the Future<br> Conclusion<br> <br> PART V: Imaging Beyond the Diffraction Limit<br> <br> SPECTROSCOPIC IMAGING OF BIOLOGICAL SAMPLES USING NEAR-FIELD METHODS<br> Tip-Enhanced Raman Scattering (TERS)<br> Detection of Biomolecules<br> Biopolymers<br> Membranes, Viruses, and Bacteria<br> Conclusion<br> <br> INFRARED MAPPING BELOW THE DIFFRACTION LIMIT<br> Introduction and Description of Early Work<br> Near-Field Microscopy by Elastic Scattering from a Tip<br> <br> PART VI: Developments in Methodology<br> <br> SUBSURFACE RAMAN SPECTROSCOPY IN TURBID MEDIA<br> Introduction<br> Techniques for Deep Noninvasive Raman Spectroscopy<br> Examples of Application Areas<br> Conclusions<br> <br> NONLINEAR VIBRATIONAL SPECTROSCOPIC MICROSCOPY OF CELLS AND TISSUE<br> Introduction<br> Principles of Nonlinear Optical Imaging<br> Instrumentation for Multimodal Nonlinear Microscopy<br> Applications<br> <br> WIDEFIELD FT-IR 2D AND 3D IMAGING AT THE MICROSCALE USING SYNCHROTRON RADIATION<br> Introduction<br> Optical Evaluation<br> Mathematical Evaluation of Hyperspectral Cubes<br> Widefield versus Raster Scanning Geometries<br> Examples<br> Conclusions<br> <br> Index

<p>“This book is an excellent addition to the bookshelves of vibrational spectroscopic imaging practitioners as well as researchers in related fields, including biology, medicine, surface analysis, process control, forensics, and others.”  (<i>Anal Bioanal Chem</i>, 10 May 2015)</p>

Reiner Salzer is Professor Emeritus of Analytical Chemistry at the Dresden University of Technology (Germany). He has authored almost 300 publications and serves on various national and international scientific boards. Professor Salzer is a member of the Norwegian Academy of Science and a recipient of the Emich Plaque of the Austrian Society of Analytical Chemistry. Recently he also received the Clemens Winkler Medal of the GDCh (German Chemical Society), the Hanus Medal of the Czech Chemical Society and the Ioannes Marcus Marci Medal of the Czech Spectroscopic Society. Professor Salzer served as President of the Division Analytical Chemistry of the GDCh and is National Delegate to the Division Analytical Chemistry of EuCheMS, where he is Head of the Study Group Education. He is Chairman of the ECTN Label Committee for the Quality Eurolabels in Chemistry Education.<br> <br> Heinz W. Siesler is Professor Emeritus of Physical Chemistry at the University of Duisburg-Essen (Germany). After receiving his PhD in chemistry from the University of Vienna (Austria), he worked as a postdoctoral fellow at the University of Cologne (Germany) and as a lecturer at the Witwatersrand University (Johannesburg, South Africa). Prior to his academic position, he gained industrial experience as section<br> head in molecular spectroscopy and thermal analysis in the Corporate R&D Department of Bayer AG (Dormagen, Germany). Between 1992 and 2010 he held guest professorships in France, Japan, and Austria. Professor Siesler is a recipient of the EAS Award, the Tomas Hirschfeld Award and the Buechi Award in near-infrared spectroscopy and he is a Fellow of the Society for Applied Spectroscopy. His main research focuses on the application of vibrational spectroscopy to chemical and polymer research, analysis and quality control, and he has authored more than 230 publications in this field.

This second edition of the successful reference work has been updated and revised with approximately<br />30% new content to reflect the numerous instrumental developments and improvements, as well as the significant expansion of this rapidly developing field. For example, the combination of IR imaging with AFM has enhanced the achievable lateral resolution by an order of magnitude down to a few hundred nanometers, thus launching a multiplicity of new applications in material science. Furthermore, Raman and IR spectroscopic imaging have become key technologies for the life sciences and today contribute tremendously to a better and more detailed understanding of numerous biological and medical research topics.<br /><br />The topical structure of this new edition is now subdivided into four parts. The first treats the fundamentals of the instrumentation for infrared and Raman imaging and mapping and an overview on the chemometric tools for image analysis. The second part describes a wide variety of applications ranging from biomedical via food, agriculture and plants to polymers and pharmaceuticals. This is followed by a description of imaging techniques operating beyond the diffraction limit, while the final part covers special methodical developments and their utility in specific fields. <br /><br />With its many valuable practical tips, this is a must-have overview for researchers in academic and industrial laboratories wishing to obtain reliable results with this method.

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